Protoplast preparation and regeneration of Rhizoctonia cerealis

摘要：

　　Rhizoctonia cerealis is the main pathogen of wheat sharp eyespot in our country.In order to obtain high quality protoplasts for the transformation of Rhizoctonia cerealis, the effect factors on protoplast preparation of R.cerealis were studied.The results showed that the suitable conditions of protoplast preparation were 6 d mycelium, in the mixture enzymes of 15 mg/mL Lywallayme and 10 mg/mL Snailase, digesting at 30℃ for 4 h, the protoplast yield reached 3.0 × 106 cell/mL.

关键词： Rhizoctonia cerealis protoplast preparation regenetation

作者: Dezhen Zhang Xiaoxia Chen Xianyue Gao Jinfeng Yu

作者单位: Department of Plant Pathology, College of Plant Protection,Shandong Agricultural University ,Tai”an Department of Plant Pathology, College of Plant Protection,Shandong Agricultural University ,Tai”an

会议类型: 国内会议

会议名称: 中国植物病理学会第十二届青年学术研讨会

会议地点: 泰安

会议语种:英文

页码: 37-37

在线出版日期: 2015-10-01（万方平台首次上网日期，不代表论文的发表时间）