Activity of antibacterial protein from maggots against Staphylococcus aureus in vitro and in vivo
Maggots (larvae of Lucilia sericata) have shown therapeutic effects on refractory wounds infected with bacteria, yet the bacterial killing mechanisms are unclear.Herein, we report the isolation and purification of an antibacterial protein from maggots (MAMP).MAMP demonstrated inhibitory activity against both standard strains and clinically isolated antibiotic-resistant strains of Staphylococcus aureus in vitro.The topical use of MAMP effectively decreased the viability of S.aureus and promoted wound healing in an S.aureus mouse skin infection model.MAMP exerted its antibacterial activity via a bactericidal mechanism based on observations using scanning electron and transmission electron microscopy.MAMP interacted with the bacterial cell membrane and disrupted the cell surface structure.In addition,MAMP had weak hemolytic activity at a high concentration.Taken together, MAMP exhibits potential use as a topical agent for treating bacterial infections.
maggots antibacterial protein Staphylococcus aureus
ZHEN ZHANG JIANGNING WANG BO ZHANG HUANRAN LIU WEI SONG JIAO HE DECHENG LV SHOUYU WANG XIAOGUANG XU
Department of Orthopedic Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian Department of Reparative and Reconstructive Surgery, Luhe Hospital, Capital Medical University, Beij Departments of Neurosurgery, The First Affiliated Hospital of Dalian Medical University,Dalian 11601 Clinical Laboratory, The First Affiliated Hospital of Dalian Medical University,Dalian 116011 Department of Neurosurgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 1160
国内会议
北京
英文
174-180
2015-10-31(万方平台首次上网日期,不代表论文的发表时间)