The nuclear factor-κB correlates with increased expression of interleukin-6 and promotes progression of gastric carcinoma
The interleukin-6 (IL-6) pathway is one of the mechanisms that link inflammation and angiogenesis with malignancy.Since nuclear factor-κB (NF-κB) is a potential sign for inflammation, NF-κB has been associated with the progression of disease in various types of cancer.In the present study, we investigated the effect of NF-κB on the IL-6 pathway in gastric carcinoma and their correlation with disease status and prognosis.The mRNA and protein levels of NF-κB, IL-6 and vascular endothelial growth factor (VEGF) were detected by western blotting and reverse transcription (RT) quantitative PCR (RT-qPCR).Using immunohistochemistry, we examined the expression of these proteins in normal and human gastric cancer tissue samples.The concentrations of IL-6 and TNF-α in collected blood samples were measured according to the enzyme-linked immunosorbent assay (ELISA).IL-6 and TNF-α were found to be expressed at high levels in human gastric cancer samples.A positive correlation was found between the expression of IL-6 and NF-κB by immunohistochemical and further correlation analysis.IL-6, NF-κB and VEGF protein and mRNA levels increased significantly in gastric cancer tissue compared with those in adjacent normal mucosa tissue samples.In conclusion, our findings demonstrate that NF-κ B, IL-6 and VEGF mRNA and protein levels increase significantly in gastric cancer tissues.In addition, the expression of NF-κB was positively correlated with the expression of IL-6 according to immunohistochemical and further correlation analysis, which suggests that the suppression of NF-κB or IL-6 may be a potential target for clinical therapy of gastric cancer in the future.
gastric cancer interleukin-6 nuclear factor-κB vascular endothelial growth factor
YEFENG YIN XIULIAN SI YAN GAO LEI GAO JIANGNING WANG
Department of Plastic and Reconstruction Surgery, Beijing Luhe Hospital,Capital Medical University, Beijing 101199, P.R.China
国内会议
北京
英文
416-420
2015-10-31（万方平台首次上网日期，不代表论文的发表时间）