A systematic quality control method of Huangqi decoction:simultaneous determination of eleven flavonoids and seven triterpenoid saponins by ultra high-pressure liquid chromatography coupled with electrospray ionization-mass spectrometry
A novelmethod of ultra high-pressure liquid chromatography coupled with mass spectrometry (UHPLC-MS) was developed for the quantitative analysis of 18 major bioactive components from Huangqi decoction (HQD).HQD is a classic traditional Chinese medicine (TCM) commonly used to treat consumptive and chronic liver diseases.Chromatographic separation was performed on a reverse-phase C18 column for 30 min at a flow rate of 1 mL/min.The optimum mobile phase for the gradient elution was 0.05% aqueous formic acid and acetonitrile.All of the analytes showed good linearity over the tested concentration ranges (r2 > 0.9972).The recoveries of the three concentration levels ranged from 91.14% to 106.21% with relative standard deviation (RSD)less than 4.69%.Intra-and inter-day precisions were less than 4.73% and 4.97%, respectively.Moreover, this method was successfully used to determine the content of HQD extracts in three different batches.Hence, this method could be used for the multi-component quality control of HQD.
Huangqi decoction bioactive components UHPLC-MS quality control
Hui-Long Luo Jie Zhong Fu-Yuan Ye Qian Wang Yue-Ming Ma Ping Liu Hua Zhang Ming-yu Sun Jian Jiang
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, Ch Instrumental Analysis Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Institute of Liver Diseases, Sh
国内会议
上海
英文
57-72
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）