Tetramethylpyrazine protects brain micro-vascular endothelial cells from oxygen-glucose-deprivation induced injury in vitro via reducing oxidative stress and down-regulating the active of Rho-kinases
We investigated the effect of Tetramethylpyrazine (TMP), one of the main active compounds isolated from Chuanxiong which has significantly neuro-protective effect, on micro-vascular endothelial cells (BMECs) damage induced by oxygen-glucose-deprivation (OGD).Recent studies have shown that Rho-kinases play a key role in the pathogenesis of ischemic stroke.As an extending study we investigated whether the neuro-protective effect of TMP is mediated by mitigating the active of Rho-kinases thereby reducing oxidative stress.It was found that the presence of TMP decreased Reactive Oxygen Species (ROS),one vital product of oxidative stress which can lead to BMECs contraction and increase its permeability, by down-regulate the active Rho-kinases in OGD-induced BMECs.Furthermore TMP maintain the integrity of cytoskeleton in OGD-induced BMECs by down-regulate RhoA-kinase protecting the integrality of blood-brain barrier (BBB).The results suggest that TMP exert neuro-protective effect in OGD-induced BMECs via down-regulating the active of Rho-kinases, reducing oxidative stress and protecting the integrality of BBB.
Tetramethylpyrazine oxygen-glucose-deprivation brain micro-vascular endothelial cells oxidative stress cytoskeleton
吴倩
安徽中医药大学 230012
国内会议
上海
英文
242-255
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）