The research of anti-tumor mechanism of ophiopogonin D on MCF-7 cells
(0) Microtubules are one of the major cytoskeletal components and play critical roles in diverse cellular processes, cell division and mitosis.It has become a successful target for anticancer therapy.Previous studies have shown that large amount of natural products or synthetic drags could interact with microtubules and interfere with the function of microtubules.Ophiopogonin D is one of the main chemical compositions of Ophiopogon japonicus which has variety of pharmacological activity.Here we measured the inhibitory effects of Ophiopogonin D on the growth of MCF-7 breast cancer cells.The experiments include MTT, Colony formation assay, cell cycle, westem-blot, Tunel, Tubulin Polymerization Assay, Immunofluorescence microscopy, Molecular docking.Our results showed that Ophiopogonin D could inhibit the growth of cells, arrest the cells in G2/M phase and induce the cell apoptosis.Further mechanism studies proved that Ophiopogonin D induced cells apoptosis by regulating protein expression of Caspase family and promoted the polymerization of tubulin.The findings of this study indicate that Ophiopogonin D may be used as a new microtubule-inhibitor.
Microtubules Ophiopogonin D G2/M phase MCF-7 cells
Qingqing Zang Lu Zhang Cheng Huang
Department Lab of Drug Discovery, School of Pharmacy, Shanghai University of Traditional Chinese Med Department Lab of Drug Discovery, School of Pharmacy, Shanghai University of Traditional Chinese Med
国内会议
上海
英文
281-295
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）