Seamless genome editing in mammalian cells using CRISPR-Cas9 system

摘要：

　　Genome editing is valuable for understanding gene function and for gene therapy.But the rate of traditionally homologous recombination is very low and the Cre/loxP leaves behind a 34bp sequence together with the targeted mutation and the transposase can reintegrate the released piggyBac into other chromosomal sites.Here we presented a seamless strategy for editing CCR5 gene using the CRISPR-Cas9 system twice based on the HR and SSA repair mechanism respectively.

作者: Yichun Bai Linjie He Kun Xu Zhiying Zhang

作者单位: Northwest A&F University, Yangling, shaanxi, 712100, China

会议类型: 国内会议

会议名称: 第九次全国动物生物技术学术研讨会

会议地点: 陕西杨凌

会议语种:英文

页码: 29-30

在线出版日期: 2015-12-23（万方平台首次上网日期，不代表论文的发表时间）