G-protein subunits Galphaq localization and mRNA expression in sheep skins associated with different coat colors
To better understand the expression patterns of Gnαq in formation of mammalian coat color, mRNA expression was detected by real-time fluorescent quantitative polymerase chain reaction (PCR);cellular localization of Gnaq in white and black skins were examined by immunohistochemistry;and qualitative investigation of Gnαq protein was analyzed by Western blot.The results showed high homology of sheep Gnαq cDNA sequence (98 %).The result was corrected by sequence analysis.In white and black sheep skins, Gnαq protein was localized to hair shaft, inner root sheath, dermal papilla and hair matrix.The amounts of Gnαq mRNA and Gnαq protein in black sheep skins were significantly greater than that of in white sheep skins (P 0.01).In conclusion, Gnαq is expressed in different coat colors.These results suggest that Gnαq is correlated with pigment formation in different coat colors.
Gnaq Sheep Skin Coat Colors
Zhen Li Jieli Lu Yiwen Zhao Quanhai Pang Xiaowei Sun
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China
国内会议
陕西杨凌
英文
53-57
2015-12-23（万方平台首次上网日期，不代表论文的发表时间）