Enhancement of CRISPR-Cas9 system mediated homologous recombination in human cells by fusion-expression of Saccharomyces cerevisiae Rad52
The genes Rad51 and Rad52 have been known as major players in the management of exogenous DNA in eukaryotic organisms and play a critical role in the choice of repair system.Previous research has shown that expression of Saccharomyces cerevisiae Rad52 in human cells resulting in an up to 37-fold increase in gene targeting by homologous recombination (HR).In addition, when a site-specific nucleases produce a double-stranded break (DSB), the frequency of HR events in the targeted locus increases significantly.To overcomeone current limitation of Cas9-induced HR which is low efficiency, in this study, the CRISPR-Cas9 mediated HR in human cells was enhanced successfully by expressing ScRad52-SpCas9 fusion and this approach offers a new tool for genome editing study.
Simin Shao Kun Xu Furong Han Zhiying Zhang
Northwest A&F University, Yangling, Shaanxi, 712100, China
国内会议
陕西杨凌
英文
65-67
2015-12-23（万方平台首次上网日期，不代表论文的发表时间）