Improving CRISPR/Cas9 multiplex editing ability by using the endoribonuclease Drosha

摘要：

　　Higher organisms, such as mammalian animals and plants, usually have a complex network to organize all their life activities.Thus multiple targeting in genome is of great meaning for both scientific research and clinical use.The bacterial type Ⅱ clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated (Cas) protein system has been engaged in introducing double-strand breaks (DSB) to a specific target.But it is still a challenge for multiple genome targeting in animals.Here we report a Drosha-based multiple-sgRNA expressing method.Using this method, we got the multiple mutations in each different targeted genome loci.Because of the universality of Drosha in eubacteria and eukaryotes, this method could make the CRISPR/Cas9 system much more efficient and applicable in genome editing.

作者: Qiang Yan Kun Xu Jiani Xing Zhiying Zhang

作者单位: College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China

会议类型: 国内会议

会议名称: 第九次全国动物生物技术学术研讨会

会议地点: 陕西杨凌

会议语种:英文

页码: 76-77

在线出版日期: 2015-12-23（万方平台首次上网日期，不代表论文的发表时间）