Skeletal Growth and IGF Levels in Rats after HT042 Treatment
HT042, a new herbal prescription consisting of Astragalus membranaceus, Phlomis umbrosa,and Eleutherococcus senticosus, is used in traditional Korean medicine to stimulate growth in children.This study was conducted to investigate the effects of HT042 on skeletal growth, insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) levels, and estrogenic activity in female rats.Female Sprague-Dawley rats were divided to the control, recombinant human growth hormone (rhGH;20 μ g/kg/day), and HT042 (100 mg/kg/day)groups and treated for 3 weeks.Axial skeletal growth,femur length, and growth plate length were measured every 3 weeks.The serum IGF-1 and IGFBP-3 levels were analyzed.Moreover, the estrogenic activity of the herbal extracts in the immature and ovariectomized rats was tested.The nose-anus, nose-tail, femur, and growth plate lengths were increased significantly in the HT042 group.IGF-1 and IGFBP-3 were highly expressed in the hypertrophic zone of the growth plate.The serum IGF-1 levels were increased.Moreover,HT042 had no uterotrophic effects in the rats.Consequently, HT042 promoted longitudinal bone growth by stimulating cell proliferation in the epiphyseal plate and inducing the expression of IGF-1 without an estrogenic response.HT042 may be helpful in stimulating growth in children with short stature.
HT042 herbal extract skeletal growth IGF-1 uterotrophic assay
Donghun Lee Gyu Tae Chang Ho-Young Choi Hocheol Kim
Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University,Hoegi-dong, Dong Department of Pediatrics, College of Korean Medicine, Kyung Hee University, Seou1130-701, Republic o
国内会议
2015·中国徐州世界中联药膳食疗研究专业委员会第六届学术年会暨第十一届国际药膳食疗学术研讨会
河南徐州
英文
89-97
2015-07-31（万方平台首次上网日期，不代表论文的发表时间）