Responses of Glutathione System in Freshwater Fish Carassius auratus to Zinc and EDTA-Zn Exposure
Laboratory experiments were carried out to determine zinc accumulation and glutathione defense system in freshwater fish Carassius auratus.Fish were subjected to different concentrations of Zn2+ and its EDTA complex (0.05, 0.1, 0.5, 1 and 2 mg/L) after 40 days of exposure.The chemical analyses showed higher zinc concentrations for Zn2+ treatments followed by Zn-EDTA.At the lowest Zn2+ or Zn-EDTA level, decreases of glutathione reductase (GR), oxidized glutathione (GSSG) content and reduced glutathione (GSH) content were observed.GSSG, GSH and tGSH (total glutathione) content were almost suppressed with Zn2+ treatments.GSH/GSSG ratio increased at the lowest Zn2+ level but decreased with higher exposure, but the ratio almost increased markedly with Zn-EDTA exposure.Glutathione antioxidant systems are sensitive to lower Zn-EDTA levels (< 0.5 mg/L).These parameters of glutathione system are valuable tools in the study of oxidative stress in aquatic ecosystems.
zinc EDTA glutathione Carassius auratus
Hui Liu Weimu Wang
Key Laboratory of Efficient Irrigation-Drainage and Agricultural Soil-Water Environment in Southern China, Ministry of Educoting, Hohai University, Nanjing 210098, China;Department of Agricultural and Biological Engineering, University of Florida,Gainesville, FL 32611, USA
国内会议
南京
英文
24-32
2015-07-25（万方平台首次上网日期，不代表论文的发表时间）