Effect of molybdenum fed along with cadmium on spleen-related gene expression in goat
In this study, the effect of molybdenum fed along with cadmium on spleen-related gene expression in goat was evaluated.Thirty-six goats weighing nearly 20kg each were randomly divided into four groups of nine goats each.Control group was drenched corresponding quantitative deionized water.Three experiment groups were drenched the same amount CdCl2 (0.5 mgCd/kg BW) firstly, and the different amount ”(NH4) 6Mo7O24 · 4H2O”(15mgMo/kg BW,30mgMo/kg BW,45mgMo/kg BW) secondly, and the three groups were represented as group Ⅰ , Ⅱ and Ⅲ, respectively.Each group was set by three replicates.Spleen tissue was collected from 3 goats of each group after they were slaughtered on 0th,25th and 50th day to measure expression of apoptosis gene including Bcl-2,Bax, Cyt C, Caspase-3, Smacand ceruloplasmin.The expression of Bcl-2, Cyt C and ceruloplasmin in each group was down-regulated significantly (P < 0.05), and Caspase-3 from each group was up-regulated significantly with the exception of group Ⅰ (P < 0.05).The Bax in group Ⅱ was up-regulated (P > 0.05), but that submitted a descending in group Ⅲ, and no statistical significance was observed on Smac.In addition, the changes in ultrastructure of spleen were detected by transmission electron microscope.Results revealed vacuoles in the lymphomacytes and apoptosis bodies of group Ⅲ.Taken together, molybdenum and cadmium could induce goat splenic cell apoptosis by involving in mitochondrial intrinsic pathway, and molybdenum and cadmium showed possible synergistic relationship, meanwhile there exists certain mechanism to repair damage from molybdenum and cadmium.
Molybdenum cadmium spleen apoptosis ultrastructure goat
GU Xiaolong CHEN Rongrong HU Guoliang ZHUANG Yu LUO Junrong ZHANG Caiying GUO Xiaoquan HUANG Aiming CAO Huabin
Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University,Nanchang 330045, China
国内会议
中国畜牧兽医学会兽医内科与临床诊疗学分会2014年学术研讨会及小动物临床技术交流会
昆明
英文
74-82
2014-07-19（万方平台首次上网日期，不代表论文的发表时间）