Induction and mechanism of HeLa cell apoptosis by 9-Oxo-10,11-dehydroageraphorone from Eupatorium adenophorum
9-Oxo-10, 11-dehydroageraphorone (euptox A), a cadenine sesquiterpene, is the main toxin from Eupatorium adenophorum.The present study aims to explore the induction and mechanism of HeLa cell apoptosis by 9-Oxo-10,11-dehydroageraphorone.The apoptosis inducing effect of the euptox A on HeLa cells was examined by MTT assay.Meanwhile, the mechanism was analyzed by flow cytometry and real-time PCR.Flow cytometry results suggest that euptox A could effectively inhibit the proliferation of HeLa cells, arrest the cell cycle transition from S to G2/M phase, can”t continue to complete the cell cycle activity (mainly form four times and mitosis), and make the cell proliferation, the QRT-PCR detection results showed that euptox A could induce apoptosis through improving the gene expression level of apoptosis proteases such as caspase-10 in HeLa cells.Its mechanism of action was probably associated with up-regulating apoptotic gene expression and arresting the cell cycle.
9-Oxo-10 11-dehydroageraphorone Eupatorium adenophorum Apoptosis Mechanism
LIAO Fei HU Yanchun WU Lei TAN Hui LUO Biao LUO Lijuan HE Yajun QIAO Yan MO Quan ZHOU Yancheng ZUO Zhicai REN Zhihua DENG Junliang WEI Yahui
Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicin Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicin Key Laboratory of Resource Biology and Biotechnology in Western China, School of Life Science ,North
国内会议
中国畜牧兽医学会兽医内科与临床诊疗学分会2014年学术研讨会及小动物临床技术交流会
昆明
英文
91-97
2014-07-19（万方平台首次上网日期，不代表论文的发表时间）