Intracellular Self-Assembly and Disassembly of 19F Nanoparticles Confer Respective ”Off” and ”On” 19F NMR/MRI Signals for Legumain Activity Detection in Zebrafish

摘要：

19F MRI has higher selectivity but lower sensitivity than 1H MRI for in vivo diagnosis.Therefore, to avoid using high injection dose of 19F probe while in the meantime maintain high sensitivity of19F MRI has remained challenging.Locally self-assembly and disassembly of19F nanoparticles could be one of the ”smart” strategies to achieve this goal.Herein, we report a dual-functional probe 1 for glutathione (GSH)-controlled self-assembly and subsequent legumain (Lgmn)-controlled disassembly of its nanoparticles (i.e., 1-NPs).Self-assembly and disassembly of 1-NPs confer 19F magnetic resonance (MR) signals ”off” and ”on”, respectively.Employing this strategy, we successfully applied 1 for consecutive detections of GSH and Lgmnin vitro and in cells, imaging Lgmn activity in HEK 293Ttumors in zebrafish at a low dosage under 14.1 Tesla.

作者: 梁高林

作者单位: 中国科学技术大学化学系,安徽省合肥市金寨路96号,230026

会议类型: 国内会议

会议名称: 国家自然科学基金委员会“可控自组装体系及其功能化”重大研究计划年度会议暨研讨会

会议地点: 厦门

会议语种:英文

页码: 239-240

在线出版日期: 2015-02-05（万方平台首次上网日期，不代表论文的发表时间）