Systematic Separation and Purification of Iridoid Glycosides and Crocetin Derivatives from Gardenia jasminoides Ellis by High-speed Counter-current Chromatography
Objective:To develop a high-speed counter-current chromatography (HSCCC) method for the systematic separation and purification of iridoid glycosides and crocetin derivatives on a preparative scale from Gardenia.Method : After fractionation using HPD100 column chromatography, n-butanol ∶ ethanol∶ water (10∶1 ∶ 10, v/v) was selected to purify gardenoside, 6β-hydroxy geniposide and geniposidic acid from fraction A; ethyl acetate∶ n-butanol∶ water (2 ∶ 1.5∶3, v/v) was used to isolate geniposide from fraction B; crocin-1, crocin-2, crocin-3 and crocin-4 were purified by hexane∶ ethyl acetate∶ n-butanol ∶ water (1 ∶ 2 ∶ 1 ∶ 5, v/v) from fraction C.The head-to-tail elution mode was used with a flow rate of 8.0mL/min and a rotary speed of 600 rpm.Results : After HSCCC isolation,151.lmg of gardenoside,52.2mg of 6β-hydroxy geniposide and 24.5mg of geniposidic acid were obtained from 800mg of fraction A;587.2mg of geniposide was obtained from 800mg of Fraction B ;246.2mg of crocin-1,34.2mg of crocin-2,24.4mg of crocin-3 and 24.7mg of crocin -4 were obtained from 1000mg of fraction C.Their purities were found by UPLC analysis to be 91.7% ,93.4% ,92.5% ,98.2% ,94.1% ,96.3% ,94.1% and 98.9% respectively.Conclusion:The present results demonstrates that the main iridoid glycosides and crocetin derivatives in Gardenia can be obtained efficiently from extracts using HSCCC.
High-speed counter-current chromatography (HSCCC) separation and purification crocetin derivative (crocin) iridoid glycoside Gardenia jasminoides Ellis
Yarong Wang Yang Chen Liang Deng Shining Cai Jia Liu Wenna Li Lianfeng Du Guozhen Cui Xin Xu Tao Lu Ping Chen Hao Zhang
国内会议
贵阳
英文
335-348
2015-12-10（万方平台首次上网日期，不代表论文的发表时间）