Development of Magnetic Separation and Quantum Dots labeled immunoassay for the detection of Lead
Immunoassays for heavy metals offer an accurate and alternative approach to traditional techniques for detection of Pb.In this study, a Pb-chelate was prepared with DTPA.The detection antigen (BTX-BSA) complex was linked to bovine serum album and the immunizing antigen (BTX-IgG) complex was linked to keyhole limpet hemocyanin (KLH).BALB/c mice were immunized with KLH-DTPA-Pb and spleen ceils from BALB/C mice were fused with Sp2/0 cells.One cell line (3F7) produced monoclonal antibodies with preferential selectivity and sensitivity for BSA-DTPA-Pb.This work presents a rapid and sensitive method for detecting Pb at low concentration.In this method, lead antibody is conjugated with magnetic beads to separate Pb from the blood and the other antibody, associated with quantum dots, is used to detect fluorescence.The Pb in blood can be quantified using the relationship between the QDs fluorescence intensity and the Pb in blood frequency following magnetic separation.The numerous advantages of detecting Pb at low concentration using the presented method include ease of preparation, low cost, fast detection,and high sensitivity.
Pb Antigen Monoclonal Antibody Immunoassay Magnetic beads Quantum dots
Hubo sun Mengmeng Wang Jilong Wang Peili Huang
Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, No.10 Xitoutiao You An Men, Beijing 100069, China;Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, No.10 Xitoutiao You AnMen, Beijing 100069, China
国内会议
北京
英文
192-204
2014-06-01（万方平台首次上网日期，不代表论文的发表时间）