Effect of BMP-7 on the expression of mesenchymal-epithelial transition markers insilicosis model
This study presented the effect of bone morphogenic protein-7 (BMP-7) inhibiting epithelial-mesenchymal transition (EMT) in silicosis model In vivo, Wistar rats were exposed to silica by intratracheal instillation and 7 days later rats were treated with BMP-7 or saline every other day.Rats were sacrificed at 15 days and 30 days after instillation to observe the expression of Vimentin, E-cadherin, TGF-β and phosphorylation-p38 MAPK in the lung tissues.Immunohistochemistry demonstrated the increasing expression of Vimentin and decreasing expression of E-cadherin, however, after intervention of BMP-7 Vimentin expression was down-regulated and E-cadherin expression was up-regulated.Western blot analysis showed the expression of TGF-β and phosphorylation-p38 MAPK in BMP-7 group were lower than that in silica group.In vitro, we found that the occurrence of EMT in A549 cells stimulated by silica could be inhibited by BMP-7.Our data showed BMP-7 inhibited EMT induced by silica associated with inhibiting TGFβ/p38 MAPK pathway.
Bone morphogenic protein-7 Epithelial-mesenchymal transition Silica p38 MAPK
Yan Wang Gengxia Yang Zhonghui Zhu Piye Niu Ai Gao Li Chen Lin Tian
Beijing Key Laboratory of Environmental Toxicology, School of Public Health, Capital Medical University, Beijing 100069, China
国内会议
北京
英文
224-238
2014-06-01（万方平台首次上网日期，不代表论文的发表时间）