Crosstalk between the Smad and JNK Pathway in the Formation of Myofibroblast in Bleomycin-induced Pulmonary Fibrosis
OBJECTIVE: To investigate the relationship between JNK and Smad pathway in the formation of myofibroblast in bleomycininduced pulmonary fibrosis.METHODS: Ninty-six male C57BL/6 mice were randomly divided into four groups: control group, model group injected with bleomycin, SP600125 group given bleomycin and SP600125, a specific inhibitor of JNK, and SB431542 group administered bleomycin and SB431542 that can inhibit the Smad pathway.Mice were randomly sacrificedsacrificed on days 7, 14 and 28 after bleomycin treatment.Histological changes in the lungs were evaluated by HE and Masson stain and scored.The content of hydroxyproline was measured by alkaline hydrolysis method, the localizations of α-SMA were determined by immunohistochemistry and the expression of pJNK, and p-Smad3 and α-SMA were measured by Western blot.
D.Li Y.F.Du L.Qian X.Y.Hao X.J.Liu
Department of Geriatrics, the First Hospital of Shanxi Medical University, Taiyuan,China
国内会议
苏州
英文
364-365
2015-09-11（万方平台首次上网日期，不代表论文的发表时间）