To evaluate the effectiveness and safety of autologous cytokine-induced killer (CIK) cells in elderly patients with diffuse large B-cell lymphoma.Peripheral blood mononuclear cells (PBMC) were isolated from nine elderly patients with diffuse large B-cell lymphoma.PBMCs were augmented by priming with interferon gamma (IFN-γ) followed by IL-2 and monoclonal antibody (mAb) against CD3.Autologous CIK cells (range 5 ×109-1 x 1010) were then infused back to individual patients; infusion was repe-ated every 4 weeks for 32 weeks (eight cycles).Patients were assessed for changes in lymphocyte subgroup,tumor-related biological parameters,imaging characteristics,the condition of remission,quality of life (QOL), and survival.Prior to CIK infusion,two patients were in complete remission and seven patients were in partial remission.After autologous CIK cell transfusions,the proportion of CD3+, CD3+CD8+,and CD3+CD56+ cells were significantly increased compared with baseline (P<0.05) ; whereas serum levels of β2-microglobulin and LDH were significantly decreased (P<0.05).The lymphoma symptoms were reduced and QOL was improved (P<0.05) in all patients.All patients achieved complete remission at study endpoint.No adverse reactions were reported.Autologous CIK cell immunotherapy is safe and efficacious for the treatment of elderly patients with diffuse large B-cell lymphoma.
Xue-chun Lu Bo Yang Rui-li Yu Xiao-hua Chi Shuai Tuo Chao-wei Tuo Hong-li Zhu Yao Wang Chao-guang Jiang Xiao-bing Fu Yang Yang Yang Liu Shan-qian Yao Han-ren Dai Li-li Cai Bing-jun Li Wei-dong Han
Institute of Otorhinolaryngology,Chinese PLA General Hospital,Beijing 100853,China Department of Pharmacy,The Second Artillery General Hospital,Beijing 100800,China Department of Ultrastructural Pathology,No.202 Hospital of Chinese PLA,Shenyang 110003,China Department of Geriatric Hematology,Chinese PLA General Hospital,Beijing 100853,China Medical Department,Chinese PLA,General Hospital,Beijing 100853,China Department of Geriatric Laboratory Medicine,Chinese PLA General Hospital,Beijing 100853,China