Butanol production by a Clostridium beijerinckii mutant with high ferulic acid tolerance
A mutant strain of Clostridium beijerinckii,with high tolerance to ferulic acid,was generated using atmospheric pressure glow discharge and high-throughput screening of C.beijerinckii NCIMB 8052.The mutant strain M11 produced 7.24 g/L of butanol when grown in P2 medium containing 30 g/L of glucose and 0.5 g/L of ferulic acid,which is comparable to the production from non-ferulic acid cultures(8.11 g/L of butanol).When 0.8 g/L of ferulic acid was introduced into the P2 medium,C.beijerinckii M11 grew well and produced 4.91 g/L of butanol.Both cell growth and butanol production of C.beijerinckii M11 were seriously inhibited when 0.9 g/L of ferulic acid was added into the P2 medium.Furthermore,C.beijerinckii M11 could produce 6.13 g/L of butanol using non-detoxified hemicellulosic hydrolysate from diluted sulphuric acid-treated corn fibre(SAHHC)as the carbon source.These results demonstrate that C.beijerinckii M11 has a high ferulic acid tolerance and is able to use non-detoxified SAHHC for butanol production.
Clostridium beijerinckii ferulic acid fermentation high-throughput screening non-detoxified hemicellulosic hydrolysate
Jun Liu Ting Guo Xiaoning Shen Hanjie Ying
College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing210009,People”s Republic of China
国内会议
上海
英文
1-11
2015-11-06（万方平台首次上网日期，不代表论文的发表时间）