Polymorphisms of the FT gene as a tool to identify underground rhizome types of bamboos
The Flowering Locus T(FT)-like genes of angiosperms are highly conserved.The FT-encoded proteins include a phosphatidylethanolamine-binding(PEBP)domain that is involved in the control of shoot apical meristem identity and flowering time.In the present study,FT genes were investigated in 20 bamboo species that are grouped into sympodial,mixed and scattered bamboos based on their morphology.All examined orthologous FT genes consisted of four exons and three introns.Their encoded protein sequences contained the critical amino acid residues Tyr85,Glu109,Leu128,Tyr134,Try138,Arg139,Gln140 and Asn152,of which each possesses a biological function.The DNA sequences were rich in single nucleotide polymorphism(SNP)sites.The SNP frequency was 1 SNP/16.8 bp,and the nucleotide diversity(π)equaled 0.265.Some SNPs altered restriction enzyme sites or resulted in changes in amino acid contents.The correlation analysis showed that several SNPs were informative in relation to the underground rhizome types of bamboos and polymorphisms of the FT gene as a tool to identify underground rhizome types of bamboos.The phylogenetic tree constructed based on the FT gene sequences showed that the obtained clustering was consistent with the underground rhizome type.The SNP markers developed in this present study will provides information for the researches of genetic diversity and molecular breeding.
Bambusoideae FT gene Single nucleotide polymorphism (SNP) underground rhizome type
Zai-en Xu Hong-jun Chen Li-fen Ji Xiao-qin Guo
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture,Zhejiang A & F University,Lin”An 311300,China
国内会议
湖南桃江
英文
193-200
2015-10-21（万方平台首次上网日期，不代表论文的发表时间）