Integration of gene expressions and DNA methylation profiles improves molecular subtype for risk assessment in Atherosclerosis
Objective: For early detection of atherosclerosis(AS),we investigated DNA methylation of AS patients and matched controls in peripheral blood in order to establish a biomarker panel potentially useful for early detection of AS.Methods and results: AS patients and matched controls(50 and 50)were recruited for peripheral blood DNA methylation analyses at the 12 genes promoter loci using nested methylation specific PCR(NT-MSP)in a test set.Based on the test set we determined the promoter methylation of TIMP-1,ABCA1,and ACAT1 as candidate biomarkers with the best sensitivity of 88%and specificity of 90%.In an independent validation set(n=100)we validated the candidate biomarkers for further use in early AS detection.In the validation set combined TIMP-1,ABCA1,and ACAT1 methylation achieved sensitivity of 88%and a specificity of 70%,with coincidence rate of 76%,respectively.Conclusions: In this first pilot study,DNA methylation patterns change significantly in AS patients and AS-related genes in peripheral blood cells.AS-specific methylation of the three-gene panel(TIMP-1,ABCA1,and ACAT1)might be a valuable biomarker for the early detection of AS.
DNA methylation profiles CpG islands Atherosclerosis
Sheng-Chao Ma Hui-Ping ZHANG Fan-Qi Kong Cheng Yang Yang-Yang He Hua Xu An-Ning Yang Xiao-Ling Yang Ju Tian Ming-Hao Zhang Yan-Hua Wang Yi-Deng JIANG Yu Zheng
Department of Physiology,West China School of Preclinical and Forensic Medicine,Sichuan University,C Department of Prenatal Diagnosis Center,General Hospital of Ningxia Medical University,Yinchuan 7500 School of basic medical sciences,Ningxia Medical University,Yinchuan 750004,China Department of Physiology,West China School of Preclinical and Forensic Medicine,Sichuan University,C
国内会议
成都
英文
1-18
2015-05-01（万方平台首次上网日期，不代表论文的发表时间）