Chimeric-gene Hairpin Structures Affect Virus Resistance to RSV and RBSV Mediated by RNA Silencing in Transgenic Rice
Two types of hairpin RNA (hpRNA) constructions were designed using a chimeric gene formed from two genes, the coat protein (CP) gene or the silencing suppressor gene, from the Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV), respectively;one type generated a single hairpin structure, whereas the other formed a two-hairpin structure.Two constructs, pRVDCPSH (double CP gene fragments, single hairpin), pRVDCPDH (double CP gene fragments, double hairpins) were individually introduced into rice plants.
Xie XY Song YZ Bi LJ Zhu CX Wen FJ
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai”an 271018, P.R.China
国内会议
沈阳
英文
260-260
2014-07-29(万方平台首次上网日期,不代表论文的发表时间)