Characterization of Small Interfering RNAs Derived from Tobacco Ringspot Virus
Plant viruses are strong inducers as well as targets of RNA silencing, and usually virus-derived small interfering RNAs (vsiRNAs) accumulate at high levels during viral infections.vsiRNA could not only degrade the viral genome to induce resistance, but also target host transcripts for posttranscriptional regulation to modulate the virus disease symptoms.In this research, small RNA libraries were constructed with tobacco leaves infected by tobacco ringspot virus (TRSV), a member of the genus Nepovirus in the family Secoviridae and the TRSV-derived vsiRNAs were characterized by deep sequencing.A total of 23 570 484 and 18 445 302 reads were sequenced from systemic leaves harvested at 5 dpi from either TRSV-or mock-inoculated tobacco plants, respectively.
Deng Congliang Zhou Qi
Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China
国内会议
沈阳
英文
292-292
2014-07-29（万方平台首次上网日期，不代表论文的发表时间）