The quantitative real-time PCR analysis of main methanogens in pit mud of different pit age

摘要：

　　Methanogens are important functional miacrobes in pit mud for the fermentation of Luzhou-flavor liquor.The aim of this work was to investigate the regularity of main Methanogens in pit mud of different pit age.This paper made quantitative analysis of Methanosaeta and Methanosarcina in pit mud with cellar age of 5, 30 and 50 years, respectively, by using real time quantitative PCR assay.The results show that the abundance of Methanosaeta and Methanosarcina increase in varying degrees with the pit age rising of pit mud.This research analyzed the copies of main Methanogens in pit mud of different pit age by using quantitative PCR assay for the first time, laid an initiai foundation for identifying good quality pit mud, and provided a theoretical basis for understanding the Mechanism of microbial fermentation of Luzhou-flavor liquor.

关键词： pit mud Methanosaeta Methanosarcina real time PCR

作者: LUO Qing-chun ZHANG Wen-xue WU Zheng-yun

作者单位: College of Light Industry,Textile and Food Engineering,Sichuan University,Chengdu 610065,China

会议类型: 国内会议

会议名称: 第二届中国白酒学术研讨会

会议地点: 无锡

会议语种:英文

页码: 151-154

在线出版日期: 2014-09-21（万方平台首次上网日期，不代表论文的发表时间）