Spatiotemporal expression of caudal-type 1 homeobox genes during 2 development of the hindgut and anorectum in human embryo
The objectives of this study were to determine the 19 spatiotemporal expression of human caudal-type homeobox genes CDX1,CDX2 and CDX4 during development of the hindgut and anorectum in the embryo and to explore the possible roles of CDX genes during morphogenesis of the hindgut and anorectum.Embryos(89)were cut into sections serially and sagittally.From gestation weeks 4-9,CDX1,CDX2 and CDX4 proteins were detected on the caudal midline by immunohistochemical staining.During week 4,extensive immunoreactivity of CDX1,CDX2 and CDX4 was detected in the dorsal urorectal septum,urogenital sinus and hindgut.From weeks 5-7,CDX1-,CDX2-and CDX4-positive cells were detected mainly in the mesenchyme of the urorectal septum and hindgut.The levels of CDX2 and CDX4 expression were lower compared to CDX1.During weeks 8 and 9,the anorectal epithelium stained positive for CDX1 and CDX4,and the anal epithelium was positive for CDX2.The CDX genes are expressed constantly during development of the hindgut and anorectum and exhibit overlapping expression patterns in the cloaca/hindgut,suggesting they are important in the morphogenesis of the human hindgut and anorectum.CDX genes might be involved in development of the anorectal epithelium after the rectum has separated from the urorectal septum.
Human Embryo CDX Hindgut Anorectum
Xiao Bing Tang Tao Zhang Wei Lin Wang ZhengWei Yuan Yu Zuo Bai
Department of Pediatric Surgery,Shengjing Hospital,China Medical University,Shenyang 110004,P.R.Chin Department of General Surgery,Affiliated Hospital of Hebei University,Baoding 071000,P.R.China The Key Laboratory of Health Ministry for Congenital Malformation,Shenyang 110004,P.R.China
国内会议
沈阳
英文
1-16
2015-04-03（万方平台首次上网日期，不代表论文的发表时间）