Garcinia xanthochymus extract protects PC12 cells from H2O2-induced apoptosis via modulation of the PI3K/AKT and NRF2/HO-1 pathways
The aim of our research was to investigate the protective effects and mechanisms of Garcinia xanthochymus, a perennial medicinal plant native to Yunnan, China, against H2O2-induced oxidative damage in rat pheochromacytoma PC12 cells.Preincubation of PC12 cells with fruit EtOAc fraction (fruit-EFr.,12.5-50μM) of G.xanthochymus for 24 h prior to H2O2 exposure markedly improvedcell viability and increased the activities of antioxidant enzymes (superoxide dismutase, catalase,and heme oxygenase-1 ”HO-1”),prevented lactate dehydrogenase release and lipid peroxidation malondialdehyde production, attenuated the decrease of matrix metalloproteinases (MMP), and scavenged reactive oxygen species (ROS).Fruit-EFr.of G.xanthochymusalso reduced BAX andcytochrome C expression and improved BCL-2 expression, thereby decreasing the ratio of BAX to BCL-2.Fruit-EFr.activated the nuclear translocation of NRF2 to increase HO-1 and induced the phosphorylation of AKT.Its cytoprotective effect was abolished by LY294002, a specific inhibitorof PI3K.Taken together, the above findings suggest that fruit-EFr.of G.xanthochymus can enhance cellular antioxidant defense capacity, at least in part, through upregulatingHO-1 expression and activating the PI3K/AKT pathway, and it suppresses H2O2-induced oxidative damage via PI3K/AKT and NRF2/HO-1 signaling pathways.
XU Jing GAN Sheng LI Jun WAND Debing CHEN Yu HU Xin YANG Guangzhong
Laboratory for Natural Products Chemistry, School of Pharmaceutical Sciences,South-Central Universit College of Chemistry and Material Sciences, South Central University for Nationalities, Wuhan 430074
国内会议
武汉
英文
1-17
2016-10-14（万方平台首次上网日期，不代表论文的发表时间）