Proteomic analysis in maize reveals the expression characteristics of proteins involved in the early grain development
The early phase of maize grain development mainly involved active cell division and enlargement,leading to increase the grain sink size for subsequent accumulation of storage material.To explore the protein synthesis, accumulation and regulation during early maize grain development, Denghai661 was used as experimental material and the middle grains were harvested after flowering artificial saturation pollination at 3, 5,10 d, respectively, and then were analyzed by isobaric tags for relative and absolute quantitation (iTRAQ)proteomics.A total of 2639 proteins were identified and quantified in maize grain, and these proteins were involved in diverse biological processes and molecular functions, of which the metabolic process and molecular processes were the main biological processes, and the catalytic activity and binding function occupied the largest molecular categories, showing that these biological processes and molecular functions played important roles in maize grain development.Quantitative analysis detected 137 proteins that were significantly differentially expressed during early maize grain development, and these proteins were mainly involved in protein, stress response, cell growth and division, carbohydrate and energy, transport, secondary, starch synthesis, transcription,signal transduetion and amino acid processes.Expression pattern clustering analysis showed that these different functional categories proteins synergy to express, which regulated the early maize grain development.Together,the results provide important biological basis for understanding the physiological and biochemical process and potential protein regulation during the early maize grain development.
Maize Grain developmental iTRAQ Proteomics Protein function
YU Tao LI Geng LIU Peng DONG Shu-ting ZHANG Ji-wang ZHAO Bin
Agronomy College of Shandong Agricultural University/State Key Laboratory of Crop Biology,Taian 271018
国内会议
山东泰安
英文
45-60
2016-10-01（万方平台首次上网日期，不代表论文的发表时间）