Acetylcholinesterase biosensor based on specific directional binding with active ligand

摘要：

　　A novel AChE biosensor based on immobilizing acetylcholinesterase on an active ligand modified electrode was developed.Using glassy carbon electrode (GCE) as working electrode, Acetylcholinesterase was successfully immobilized on the electrode by the specific directional binding with the active ligand.The biosensor enjoyed good sensitivity to the substrate acetylthiocholine chloride.It was found that linear relationship between the oxidative peak current and the concentration of acetylthiocholine chloride was found in the range of 1×10-6-3.75 ×10-5 mol · L-1.

关键词： Acetylcholinesterase Enzyme sensor Active ligand

作者: LI Yuan-qing DIAO Jian-xiong SUN Ying

作者单位: College of Resources and Environment, China Agricultural University, Beijing 100093,China

会议类型: 国内会议

会议名称: 中国化学会第十八届全国有机分析及生物分析学术研讨会

会议地点: 上海

会议语种:英文

页码: 174-176

在线出版日期: 2015-04-17（万方平台首次上网日期，不代表论文的发表时间）