Nano-Magnetic Immunosensor Based on Staphylococcus Protein A and the Amplification Effect of HRP-Conjugated Phage Antibody
In this research, super-paramagnetic Fe304 nanoparticles (magnetic particles) were coated with Staphylococcus protein A (SPA) and coupled with polyclonal antibody (pcAb) to construct magnetic capturing probes, and HRP-conjugated phage antibody was then used as specific detecting probe to design a labeled immunosensor for trace detection of Staphylococcus aureus enterotoxin B (SEB).The linear detection range of the sensor was 0.008~125 μg/L, the regression equation was Y =0.487X + 1.2 (R =0.996, N =15, p < 0.0001), the limit of detection (LOD) was 0.008 μg/L, and the limit of quantification (LOQ) was 0.008 μg/L.HRP-conjugated phage antibody, SPA and magnetic particles can enhance the sensitivity 4-fold, 3-fold and 2.6-fold higher, respectively.Compared with conventional double-antibody sandwich ELISA, the detection sensitivity of the sensor was 31-fold higher resulting from the integrated amplifying effect.The immunosensor integrates the unique advantages of SPA-oriented antibody as magnetic capturing probe, HRP-conjugated phage antibody as detecting probe, magnetic separation immunoassay technique, and several other advanced techniques,so it achieves high sensitivity, specificity and interference-resistance.It is proven to be well suited for analysis of trace SEB in various environmental samples with high recovery rate and reproducibility.
Staphylococcus protein A HRP-conjugated phage antibody signal amplification nanomagnetic immunosensor Staphylococcus aureus enterotoxin B
Xihui Mu Zhaoyang Tong Qibin Huang Bing Liu Zhiwei Liu Lanqun Hao Jinping Zhang Chuan Gao Fenwei Wang
Research Institute of Chemical Defence, State Key Laboratory of NBC Protection for Civilian,Beijing 102205, China
国内会议
广州
英文
129-142
2015-05-01（万方平台首次上网日期，不代表论文的发表时间）