The association of Hsp90 expression induced by aspirin with anti-stress damage in chicken myocardial cells

摘要：

　　The protective effect of aspirin during exposure to heat stress in broiler chickens was investigated.We assayed the pathological damage,expression and distribution of Hsp90 protein and the hsp90 mRNA expression in chicken heart tissues after oral administration of aspirin following exposure to high temperature for varying times.Heat stress induced increases in plasma aspartate aminotransferase,creatine kinase and lactate dehydrogenase activities,and caused severe heart damage,characterized by granular and vacuolar degeneration,nuclear shrinkage and even myocardium fragmentation in cardiac muscle fibers.After aspirin administration,myocardial cells showed fewer pathological lesions compared with broilers only treated by heat.A high positive Hsp90 signal was always detected in the nuclei of myocardial cells administrated with aspirin,while in myocardial cells treated with heat alone,Hsp90 in the nuclei decreased,along with a decrease in the cytoplasm.Aspirin could induce rapid and significant synthesis of Hsp90 before and at the initial phase of heat stress,and significant expression of hsp90 mRNA was stimulated throughout the whole process of experiment,compared with cells exposed to heat stress only.Thus,the specific pre-induction of Hsp90 in cardiovascular tissue was useful preparation for resisting heat-stress damage,characterized by stable damage-relative enzymes and fewer pathologic changes.

关键词： Aspirin Hsp90 Heat-stress damage Chicken Heart

作者: Xiao-hui Zhang Huai-sen Zhu Zhuang Qian Shu Tang Di Wu N. Kemper J.Hartung J.Hartung

作者单位: College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China Institute for Animal Hygiene,Animal Welfare and Farm Animal Behaviour,University of Veterinary Medic

会议类型: 国内会议

会议名称: 中国畜牧兽医学会兽医病理学分会第二十一次学术研讨会暨中国病理生理学会动物病理生理学专业委员会第二十次学术研讨会

会议地点: 太原

会议语种:英文

页码: 271-289

在线出版日期: 2015-07-24（万方平台首次上网日期，不代表论文的发表时间）