Fingerprint analysis of Clerodendranthus Spicatus from different regions by high-performance liquid chromatography
Aim: To develop a novel, simple and accurate fingerprint method for the quality control of Clerodendranthus Spicatus from different regions by high-performance liquid chromatography, which is widely used for urolithiasis.Methods: Chromatographic separation was performed on Agilent SB-C18 (250 mm×4.6 mm, 5 μm) by the gradient elution of 0.1% H3PO4 in water and acetonitrile at 0.8 mL·min-1 at 210 nm by DAD detector.The column temperature was set at 30 ℃.Results: The method has good stability and reproducibility with RSD less than 2%.10 peaks were selected as the common peaks.Rosmarinic acid was taken as a reference.The similarity of 10 batches was above 0.90.Conclusion: This method is simple and specific, which provided an effective way to assess the quality of Clerodendranthus Spicatus.
Clerodendranthus Spicatus fingerprint high-performance liquid chromatography (HPLC) quality control
Qiu Ming-feng
School of Dai Medicine, West Yunnan University of Applied Science, Jinghong, 66100,China;School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China;Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Jinghong 666100, China
国内会议
贵州兴义
英文
126-139
2016-08-25（万方平台首次上网日期，不代表论文的发表时间）