Fuyuan decoction enhances SOX9 and COL2A1 expression and Smad2/3 phosphorylation in IL-1 β-activated chondrocytes
Fu Yuan decoction (FYD), an herbal formula in China, has been widely used for osteoarthritis (OA) treatment.Herein, we determined the effects of FYD on the expression of transcription factor SOX9 and its target gene collagen type Ⅱ, alpha 1 (COL2A1) as well as the activation of Smad2/3 in interleukin (IL)-1 β-stimulated SW1353chondrosarcoma cells.Serum-derived FYD (FYD-CS) was prepared for treating SW1353 cells without or with SB431542, a TGF-β 1 receptor inhibitor.Cell cycle progression was tested by flow cytometry.The expression of SOX9 and COL2A1 and the activation of Smad2/3 (p-Smad2/3) were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and/or Western blot.The results showed that after treatment, FYD-CS,while inducing S phase cell cycle arrest, enhanced cell proliferation and protected the cells against IL-1 β-and/or SB431542-induced cell growth inhibition.Furthermore,FYD-CS reversed the decreased expression of COL2A1 and SOX9 induced by IL-1 β and SB431542,and blocked the decreased phosphorylation of p-Smad2/3 induced by IL-1 β alone or in combination with SB431542.Our results suggest that FYD promotes COL2A1 and SOX9 expression as well as Smad2/3 activation in IL-1β-induced chondrocytes, thus benefiting cell survival.
Osteoarthritis Fu Yuan decoction TGF-β signaling SOX9 collagen type Ⅱ,alpha
Yudi Zhang Rongheng Li Yu Zhong Sihan Zhang Lingyun Zhou Shike Shang
Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqin Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqin Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqin
国内会议
杭州
英文
333-341
2015-10-30（万方平台首次上网日期，不代表论文的发表时间）