Antitumor Activity of Tenacissoside H on Nude Mice Transplanted with EC9706 Cells of Esophageal Cancer through Regulating
OBJECTIVE: The purpose of the study was to elucidate molecular evidence of Tenacissoside H (TDH) inhibiting esophageal carcinoma infiltration and proliferation.METHODS: EC9706 cells were inoculated subcutaneously in right forelimbs of nude mice.Mice in negative model group were treated with saline solution.Mice in positive control group were treated with 5FU.Mice in medicinal groups were treated with TDH.All mice took administration for 3 weeks and were observed for another 3 weeks continuously.All mice were killed for isolation of tumor tissue, inhibition ratio of tumor volume and weight measured.PCNA expression was examined with immunohistochemistry.Protein expression in PI3K/Akt-NF-κB signaling pathway was determined through western blotting assay.RESULTS: TDH inhibits tumor growth greatly, relative tumor Volume being (7.46±2.67), showing significantly difference from negative model group (P<0.05).TDH induced tumor tissues necrosis.Positive rate of PCNA expression aslo decreased significantly, showing statistical difference with the negative model group (P<0.05).In western blotting assay, TDH strongly down-regulated EGFR, PI3K, Akt, p-Akt and NF-κB expression, showing statistical differences (P<0.05) from those in negative model group.CONCLUSION: TDH inhibited esophageal carcinoma infiltration and proliferation in vivo.Concrete mechanism related to inhibition on PCNA of transplanted tumors in nude mice and regulation on PI3K/Akt-NF-κB transduction cascade.
Tenacissoside H esophageal carcinoma EC9706 cells PI3K/Akt/NF-κB PCNA
贾永森 胡雪琴 Hegyi Gabriella 秦丽娟 Nora Meggyeshazi
华北理工大学中医学院,河北唐山,063000 浙江中医药大学骨伤研究所,浙江杭州,310053 匈牙利佩奇大学,补充替代医学系,匈牙利佩奇,7622 华北理工大学基础医学院,河北唐山,063000 匈牙利森美维斯大学,病理实验肿瘤研究部,匈牙科布达佩斯,1085
国内会议
中华中医药学会第七次全国中医方证基础研究与临床应用学术研讨会
乌鲁木齐
英文
96-104
2016-07-01（万方平台首次上网日期，不代表论文的发表时间）