The establishment of cognitive impairment rat models induced by chronic poisoning of organophosphate
Objective: To explore the reasonable administration way and dose of omethoate in cognitive impairment rat models induced by chronic poisoning of organophosphate.Methods: All rats were daily administered omethoate by gavage way (G) and subcutaneous injection way (S) with low, medium and high doses.The general condition and survival situation of rats in each group were observed during the experimental period.The memory reappearance test was operated by Y-electric maze after the model established.The morphology and structure of hippocampus and liver were observed by HE staining.Results: During the experiment, rats in each exposure group showed mild poisoning symptoms.The poisoning symptoms of rats in group G3 and S3 were most obvious, followed by group G2 and S2 and mildest in group G1 and S1.In group G1, 7 rats died with survival rate of only 30％ and 5 rats died with survival rate of 50％, so group G3 and S3 (10mg/kg) did not suitable for establishing models.The results of the memory reappearance test showed that the memory keeping ability (MKA) of rats in each exposure group declined at different degrees, and rats in group S2 (5mg/kg) for 4 weeks were more obviously.HE straining showed that liver and hippocampus in each exposure group was damaged at different degrees compared with normal saline group.The denatured cell index (DCI) showed that rats in group S1 (2.5mg/kg) had the mildest damage to liver, while in group S2 (5mg/kg) had the medium damage to hippocampus.Conclusion: According to the synthetical analysis for general situation, survival rate,MKA and DCI of liver and hippocampus, Group S2, subcutaneous injection with 5mg/kg each day for 4 weeks was one of the reasonable administration way and dose to establish cognitive impairment model by chronic poisoning of omethoate in rats.
chronic omethoate cognitive impairment model memory rats
Guangyi Liu Kun Ding Lixia Rong Li Zhao Meizeng Zhang
Institute of Cerebrovascular Diseases, Department of Neurology, Affiliated Hospital of Qingdao University,Qingdao 266003, China
国内会议
第二届中国针灸学会针灸康复专业委员会第二次学术会议、第三届山东中西医结合学会康复医学专业委员会第一次学术会议、第一届山东省护理学会康复护理专业委员会第一次学术会议
济南
英文
440-447
2014-10-01（万方平台首次上网日期，不代表论文的发表时间）