ZJXG decoction promoted the fracture healing by reducing the decomposition of BMP-7, IGF-1, bFGF and TGFβ1 in fracture rats
The aim is to investigate the effect of Zhuang Jin Xu Gu (ZJXG) decoction on serum levels of bone morphogenetic protein-7 (BMP-7), insulin-like growth factor-1 (IGF-1), basic fibroblast growth factor (bFGF) and transforming growth factor β 1 (TGFβ1) and to explore its promoting femoral fracture healing in rats.Femur fractures were generated in 72 male adult Wistar rats by cutting femur transversely at middle point.ZJXG decoction was administered orally after surgery for 28d.The fracture healing process was analyzed by X-ray, gross anatomy and hematoxtlin-eosin (HE) staining.The serum levels of BMP-7, IGF-1, bFGF and TGFβ1 were detected by enzyme linked immunobsorbent assay (ELISA).X-ray imaging and gross anatomy indicated that the fibrous callus tissue at the femoral fracture-end increased and the fracture line became fuzzy at 7-14 d following treatment with ZJXG Decoction.HE staining showed that the fibrous-granular tissue at the fracture-end changed gradually to fibrous, cartilaginous and osseous callus tissues from 7d to 28d.ELISA results showed that the serum levels of BMP-7, IGF-1, bFGF and TGFβ1 increased significantly 28 days following treatment with ZJXG decoction, compared to model group.It is concluded that ZJXG decoction could enhance the fracture healing by reducing the decomposition of BMP-7 and IGF-1, bFGF and TGFβ 1 and enhancing their activities.
ZJXG decoction fracture X-ray BMP-7 IGF-1 bFGF TGFβ1 rats
Zhen Zhou Chi Ning Guangwen Yang Guanxi Wang Yunliang Guo
Institute of Integrative Medicine, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003,China
国内会议
第二届中国针灸学会针灸康复专业委员会第二次学术会议、第三届山东中西医结合学会康复医学专业委员会第一次学术会议、第一届山东省护理学会康复护理专业委员会第一次学术会议
济南
英文
448-455
2014-10-01（万方平台首次上网日期，不代表论文的发表时间）