The neuropotective mechanism and the best therapeutic dose and time window of picroside Ⅱ for cerebral ischemic injury in rats
This study aims to explore the effect of picroside Ⅱ on the expression of matrix metalloproteinase-9 (MMP-9) and optimize the therapeutic dose and time window after cerebral ischemic injury in rats.The forebrain ischemia models of Wistar rats were randomly grouped according to orthogonal experimental design of ”L16(45)” and treated by injecting picroside Ⅱ intraperitoneally with different doses at various times.The morphology and ultrastructure of brain tissue were observed by haematoxylin eosin (HE) staining.The expression of MMP-9 was determined by immunohistochemical (IHC) staining and Western blotting (WB).The expression of MMP-9 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR).The Results indicated that the expression of MMP-9 was reduced obviously and the structure of brain tissue was improved obviously than those in model group after treated by picroside Ⅱ.The optimal therapeutic dose and time window of picroside Ⅱ in treating cerebral ischemic injury were ischemia 2.0h with 10mg/kg body weight according to the results of HE and IHC staining, ischemia 1.5h with 10mg/kg according to WB and ischemia 1.5h with 20mg/kg according to RT-PCR results respectively.It is concluded that picroside Ⅱ could protect the blood brain barrier and brain tissue against ischemic injury by reducing the expression of MMP-9 after cerebral ischemic injury.Based on the orthogonal experimental results, the best therapeutic dose and time window of picroside Ⅱ should be injected intraperitoneally with 10-20mg/kg body weight at cerebral ischemia 1.5-2.0h.
picroside Ⅱ cerebral ischemia therapeutic dose time window MMP-9 rats
Fangfang Pang Lixia Rong Meizeng Zhang Wei Sun Yunliang Guo
Department of Neurology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Department of Pharmacy, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, C Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University Medical College, Qi
国内会议
第二届中国针灸学会针灸康复专业委员会第二次学术会议、第三届山东中西医结合学会康复医学专业委员会第一次学术会议、第一届山东省护理学会康复护理专业委员会第一次学术会议
济南
英文
463-473
2014-10-01（万方平台首次上网日期，不代表论文的发表时间）