Electroacupuncture upregulates SIRT1-dependent PGC-1α expression in SAMP8 mice
(0) Background: Abnormalities of brain energy metabolism are involved in Alzheimer”s disease (AD).Sirtuin 1 (SIRT1) is a class Ⅲ histone deacetylase and activates peroxisome proliferator-activated receptor-γ, co-activator-1α (PGC-lα), which enhances mitochondrial biogenesis and energy homeostasis.Electroacupuncture (EA) has been reported to improve brain energy metabolism in AD.However, the effect of EA on SIRT1 and PGC-1α in AD remains unclear.Material/Methods: ATP levels were measured using assay kits in the hippocampus and frontal cortex of senescence-accelerated mouse prone 8 (SAMP8) mice.Western blotting analysis and quantitative real-time RT-PCR were performed to measure the expression of SIRT1 and PGC-1α in the hippocampus of SAMP8 mice.PGC-1α acetylation was analyzed using immunoprecipitation.Results: Compared with senescence-accelerated resistant mice 1 (SAMR1) mice, SAMP8 mice had a decline in ATP levels and the expression of SIRT1 and PGC-1α.EA treatment improved ATP levels, upregulated the expression of SIRT1 and PGC-1α, and decreased PGC-1 α acetylation.Conclusions: These data suggest that EA improved brain energy metabolism, potentially associated with the upregulation of SIRT1-dependent PGC-1 α expression.
Alzheimer”s disease Electroacupuncture Brain energy metabolism SIRT1 PGC-1α
Weiguo Dong Wanqing Guo Feng Wang Changzheng Li Yongcai Xie Xueha Zheng Hong Shi
Department of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional The Third People”s Hospital of Fujian Province, Fuzhou, Fujian, P.R.China
国内会议
郑州
英文
137-152
2016-06-03（万方平台首次上网日期，不代表论文的发表时间）