In vitro induction and morphological characteristics of autooctoploid plants in Pogostemon cablin

摘要：

　　This paper describes an efficient colchicine-mediated technique for the in vitro induction of octoploids in Pogostemon cablin and its confirmation by flow cytometry and chromosome count.The optimum concentration of colchicine treatment for octoploid induction was 0.05% for 72 h.The chromosome number of octoploid seedlings was 2n = 8x = 128.Colchicine-induced tetraploids remained stable after 6 months in soil.33 lines of autooctoploid plants were obtained.Colchicine-induced octoploids remained stable after 6 months in soil.Leaf characteristics of tetraploids and octoploids in P.cablin were compared.It was determined that leaf sizes and stomatal sizes of transplants were suitable parameters for identifying putative octoploids in P.cablin.

关键词： Pogostemon cablin (Blanco) Benth cluster buds colchicines leaf characteristic octoploidy

作者: YAN Han-Jing XIONG Yang HE Meng-Ling ZHANG Hong-Yi

作者单位: School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China

会议类型: 国内会议

会议名称: 中国生态学学会中药资源生态专业委员会第五次全国学术研讨会暨世界中医药联合会药用植物资源利用与保护专业委员会第二届学术年会

会议地点: 广州

会议语种:英文

页码: 198-202

在线出版日期: 2014-12-01（万方平台首次上网日期，不代表论文的发表时间）