Conditional Knockouts Generated by Engineered Endonuclease Reveal Roles of Coronin in C.elegans Neural Development

摘要：

Animals with conditional gene knockouts are valuable tools for studying mechanisms underlying cell and developmental biology.We have developed a conditional knockout strategy by spatiotemporally manipulating the expression of an RNA-guided DNA endonuclease,CRISPR-Cas9,in C.elegans somatic cell lineages.We showed that somatic CRISPR-Cas9 technology offers the highest possible speed to efficiently create conditional knockouts in various cell types at different developmental stages.

作者: Guangshuo Ou

作者单位: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University,Beijing 100084, China

会议类型: 国内会议

会议名称: 第三届“模式生物与人类健康”发育遗传学全国学术研讨会

会议地点: 厦门

会议语种:英文

页码: 33-33

在线出版日期: 2014-07-17（万方平台首次上网日期，不代表论文的发表时间）