Different Delivery Ways of dsRNA Expressed by Bacteria have Different Abilities to Protect Tobacco from Co-Infection with PVY and TMV
RNA interference (RNAi) is a plant self-resistant reaction and regulatory mechanism that has achieved great progress in recent years.Prokaryotic expression of dsRNA can induce RNAi, conferring the plant resistance to virus.Plants were usually infected by various viruses in their grown process.In this study, two hotspot sequences of Tobacco mosaic virus (TMV) and Potato virus Y (PVY) genomes were selected and six hpRNA vectors of single-viral or two-viral chimeric genes were constructed.The target dsRNA expressed by inducting, and then the resistant effect of the mixture dsRNA A (derived from two kinds of single-viral genes)was compared to the effect of corresponding dsRNA B (derived from one kind of two-viral chimeric gene) in different delivery ways.The data showed that dsRNA expressed by bacteria with two delivery ways could protect tobacco from co-infection with PVY and TMV, the resistant efficiency of the mixture dsRNA A was stronger than the effect of corresponding dsRNA B, the best resistant effect was 42.43%.Northern blot analysis of siRNA showed that the resistance was indeed an RNA-mediated virus resistance.
bacterially expression dsRNA different delivery ways PVY TMV
SUN Zhao-Nan XIE Xue-Ying SUN Run-Ze ZHU Chang-Xiang WEN Fu-Jiang
State Key Laboratory of Crop Biology,Shandong Agricultural University,Tai”an,Shandong 271018,China
国内会议
青岛
英文
246-250
2012-07-20（万方平台首次上网日期，不代表论文的发表时间）