Cryptotanshinione inhibits β-amyloid aggregation and protects damage from β-amyloid in SH-SY5Y cells
The deposition of amyloid β-protein (Aβ) fibrils into plaques within the brain parenchyma and along cerebral blood vessels is a hallmark of Alzheimer”s disease (AD).Aβ42 oligomers and fibrils cause the breakdown of neural circuits,neuronal death and eventually dementia.Drugs that inhibit Aβ42 aggregation may be a novel direction in AD drug discovery.Cryptotanshinone (CTS),an active component of the medicinal herb Salvia miltiorrhiza,has been shown to improve learning and memory in several pharmacological models of AD.However,the effects of CTS on the Aβ aggregation and toxicity are unclear.The current work shows the effectiveness of CTS on the inhibition of Aβ42 aggregation and toxicity to human neuroblastoma cells.In this study,we demonstrated that CTS can inhibit Aβ42 spontaneous aggregation using thioflavin T fluorescence assay and transmission electron microscopy.Furthermore,we investigated the effects of CTS on Aβ-induced oxidative cell death in cultured SH-SY5Y cells.MTT and lactate dehydrogenase assays showed that CTS reduced the cytotoxicity induced by Aβ42.CTS also dramatically reduced Aβ42-induced cellular apoptosis and increased level of reactive oxygen species (ROS) in these cells.Our study suggests that CTS may be useful in the inhibition or prevention of AD development and progression.
Cryptotanshinone Aβ aggregation apoptosis
Zhengrong Mei Pengke Yan Bing Situ Yonggao Mou Peiqing Liu
The Third Affiliated Hospital of Guangzhou Medical College,Guangdong Province 510150,PR China Department of Neurosurgery,Cancer Center,Sun Yat-sen University,Guangzhou 510060,PR China Laboratory of Pharmacology and Toxicology,School of Pharmaceutical Sciences,Sun Yat-sen University,G
国内会议
广州
英文
144-157
2013-01-12（万方平台首次上网日期，不代表论文的发表时间）