Sophoridine Exerts An Anti-Colorectal Carcinoma Effect Through Apoptosis Induction In Vitro And In Vivo
Aims-To further investigate the anti-colorectal carcinoma (CRC) effect of Sophoridine (SRI) which is a quinolizidine alkaloid extracted from a traditional Chinese medicine (TCM) Sophora alopecuroides L.and detect the mechanism involved,provide some basis for the development of Sophora alopecuroides L..Main methods-The anti-proliferation of SRI in human colorectal cells SW480 were detected by MTT assay.The potential mechanism of anti-proliferation was also investigated using apoptosis assays.The rate of apoptosis cells was detected also.The apoptosis-related proteins caspase-3,caspase-7,caspase-9,and PARP were determined by western blotting analysis.In animal studies,nude mice were subcutaneously injected with SW480 cells in the armpit to establish the xenograft tumors and administrated with different drugs (control,5-Fu,SRI H,and SRI L).The general state of health of the mice and the growth of tumors were observed and the inhibitory rate was calculated.The pathology and ultrastructure of xenograft tumors treated with SRI were observed also.Key findings-SRI significantly inhibited the growth of SW480 cells,and the administration of SRI significantly inhibited the growth of xenograft tumors without apparent toxicity.SRI”s mechanism of action involved the induction of apoptosis.Signicance-These results suggest that SRI produces obvious anti-tumor effects in vitro and in vivo.It supports the viability of developing SRI as a novel therapeutic prodrug for CRC treatment,as well as providing a method for identifying new anti-tumor drugs in TCM.
Sophora alopecuroides L. Sophoridine Apoptosis Nude Mice Traditional Chinese medicine
Lei Liang Xiao-Yan Wang Xu-Hui Zhang Bo Ji Hua-Cheng Yan Hong-Zhu Deng Xin-Rong Wu
Department of Pharmacy,Guangzhou General Hospital of Guangzhou Military Command,Guangzhou,510010,Chi Department of Pathology,School of Basic Medical Sciences,Southern Medical University,Guangzhou,51051 Department of Oncology,The 2nd People”s Hospital of Guangdong Province,Guangzhou,510317,China School of Traditional Chinese Medicine,Southern Medical University,Guangzhou,510515,China
国内会议
广州
英文
158-178
2013-01-12（万方平台首次上网日期，不代表论文的发表时间）