The effects of RNAs on gene expression and their potential application value in arteriosclerosis gene therapy
Objective To study the mechanisms of RNAs regulating gene expression and to propose the strategies of arteriosclerosis gene therapy.Methods HeLa cells were cotransfected with modulator plasmids(derived from pcDNA3.1 vector containing different length regulating sequences that produce RNAs)and reporter plasmids(derived from pEGFP-C1 vector);HeLa cells were transfected with plasmids that possess different sequences of downstream or adjacent genes of GFP reporter gene.Results We found that long inserting sequences of modulator plasmids induced stronger GFP gene activation than short inserting sequences.Changing of downstream sequences of GFP gene induced significant effects on GFP gene expression.Short sequences of adjacent genes of GFP activated GFP gene.According to the works of cis elements and trans-acting factors of RNAs conducted by our laboratory,we proposed RNA population model and strategies of arteriosclerosis gene therapy.Conclusion The length,sequence and producing position of RNAs are important factors for RNA regulating gene expression.The arteriosclerosis involves in gene expression changes.According to our experiment and theory of RNA population model,chromatin conformation and RNA population are the important material bases for gene expression regulation,so gene therapy via using RNAs can be effective strategies for the treatment of atherosclerosis.Because of the difference of human genome with animal genomes,the effectiveness of the human gene therapy agents cannot be evaluated using conventional animal models.In this study,we proposed the urgency of evaluating huamn gene therapy agents via using whole gene humanized animal model.
RNA length RNA position gene expression RNA population humanized animal model
Zhanjun Lv Zhihong Ma Jianjun Cheng Xiasnglong Kong Xiufang Wang
Department of Genetics,Hebei Medical University,Hebei Key Lab of Laboratory Animal,Shijiazhuang,Hebe Clinical Laboratory,The Second Hospital of Tangshan,Tangshan,Hebei Province 063000 Department of Immunology,Hebei North University,Zhangjiakou,Hebei Province 075000
国内会议
太原
英文
44-64
2014-08-19（万方平台首次上网日期，不代表论文的发表时间）