Promotion of quality control of Radix Isatidis based on hydrophilic interaction liquid chromatography
The current quality control(QC)of Radix Isatidis(R.Isatidis)by the fingerprint analysis is dominantly relied on reversed-phase liquid chromatography(RPLC),which may fail to provide sufficient information of many polar components.In this study,hydrophilic interaction liquid chromatography(HILIC)was introduced to promote the QC of R.Isatidis.We first analyzed 16 batches of samples with ultra-performance liquid chromatography tandem mass spectrometer,comparing traditional RPLC with HILIC.The resulting data of the two fingerprints were analyzed using analysis of similarity and retention behavior,showing that HILIC can increase the fmgerprint coverage for RPLC analysis.Then,an anti-viral assay ofR.isatidis was conducted to evaluate its efficacy.The fingerprint-efficacy relationships between the two fingerprints and anti-viral activity were investigated with principal component regression analysis.The results showed that the anti-viral activity correlated better with HILIC fingerprint(R=0.9638,P=0.016)than with RPLC fingerprint(R=0.7804,P=0.0003).This study indicated that HILIC fingerprint could better reflect the quality ofR.Istidis,which might be used to promote the QC of herbal medicines.
Hydrophilic interaction chromatography (HILIC) Reversed-phase liquid chromatography (RPLC) Quality control Fingerprint analysis Radix Isatidis Anti-influenza virus activity
Cong-en Zhang Dan Yan Yin Xiong Dan Gao Le-le Zhang Li-na Ma Cheng Peng Xiao-ping Dong
College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Military Military Institute of Chinese Materia Medica,302th Military Hospital of China,Beijing 100039,China;N Military Institute of Chinese Materia Medica,302th Military Hospital of China,Beijing 100039,China College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China
国内会议
成都
英文
127-142
2013-11-01（万方平台首次上网日期，不代表论文的发表时间）