Paeoniflorin Protects Against ANIT-Induced Cholestasis by Ameliorating Oxidative Stress in Rats
Paeoniflorin,one of the primary bioactive components in Chi shao,are widely used in traditional Chinese medicine.A lot of evidences suggest that Paeoniflorin has potential anti-oxidant effects.However,whether Paeoniflorin plays roles in cholestasis is unclear.In this study,we examined the protective effect of Paeoniforin against alpha-naphthylisothicaynate(ANIT)-induced cholestasis in rats.Our data demonstrated that the high(0.2 g/kg body weight)and medium(0.lg/kg body weight)doses of Paeoniflorin significantly prevented ANIT-induced changes in bile flow and the serum levels of total bilirubin,direct bilirubin,total bile acid,γ-glutamyltranspeptidase,glutamate-pyruvate transaminase,glutarnate-oxaloacetic transaminase and alkaline phosphatase.Moreover,we also found that Paeoniflorin significantly inhibited nitric oxide and malondialdehyde production,and restored glutathione decrease induced by ANIT.EPR data further indicated that Paeoniflorin inhibited ANIT-mediated reactive oxygen species(ROS)production.The overexpression of NADPH oxidase 4 induced by ANIT were significantly reversed when treated with Paeoniflorin,suggesting that Paeoniflorin could scavenge ROS via inhibiting NADPH oxidase 4 expression.Paeoniflorin treatment could also relieve ANIT-induced liver pathological injuries as indicated by histological assay.These findings indicate that Paeoniflorin exerts a dose-dependent protective effect on ANIT-induced cholestatic liver injury in rats,and the mechanism of this activity is related to its attenuation of oxidative stress in liver tissue.
Paeoniflorin rats α-naphthylisothiocyanate (ANIT) reactive oxygen species (ROS)
LIU tian-tian ZHAO yan-ling LIU shu-xian SUN xiao-jiao JIA lei ZHOU guang-de WANG jia-bo LI Rui-sheng SHAN li-mei XIAO xiao-he
302 Hospital of People”s Liberation Army,Beijing,100039,China;Pharmacy College,Chengdu University of Pharmacy College,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China School of Chinese Medicine,The University of Hong Kong,Hong Kong,999077,China 302 Hospital of People”s Liberation Army,Beijing,100039,China
国内会议
成都
英文
405-419
2013-11-01（万方平台首次上网日期，不代表论文的发表时间）