Development And Research On Anti-bacterial Activity Of A Novel Antimicrobial Peptide Based On Microcalorimetry
The anti-bacterial activity of a kind of novel peptide from Plutella xylostella(pxCECA1),on methicillin resistant staphylococcus aureus(MRSA)and Escherichia coli(E.coli)growth were investigated by microcalorimetry.The heat flow power(HPE)-time curves of MRSA and E.coli growth in the presence of pxCECA1 were measured using the 3114/3115 Thermal Activity Aonitor(TAM)Air Isothermal Calorimeter,ampoule mode,at 37 ℃.Some parameters such as growth rate constant k,maximum power output Pmax,total heat Output Qt,generation time tg,growth inhibitory ratio I and half-inhibitory concentration of the.drugs IC50,were obtained by analyzing the(HPE)-time curves to elucidate the antibacterial activity of pxCECA1.The.results showed that k,Pmax and Qt decreased,but I and tg increased or delayed with increasing concentration of pxCECA1.The IC50of pxCECA1 on E.coli was 5.766 μg.mL-1 and MRSA was 7.684 μg·mL-1.It could be drawn a conclusion that pxCECA1 had stronger inhibitory effect on E.coli than MRSA.In vivo test was simultaneously performed using an E.coli and MRSA infection model to validate the anti-bacterial activity ofpxCECA1.The results revealed that pxCECA1 with broad spectrum antimicrobial activity hopefully represented a.kind of promising substitute of antimicrobial agents.
Plutella xylostella pxCECA1 Anti-bacterial activity Microcalorimetry Antimicrobial peptides
He Xu Ye Wu Shi-xiao Jin Ye Wang Jin Han Xiao-lin Meng Hai-long Yuan
Department of Pharmacy,302 PLA Hospital of China,Beijing 100039,China;School of Pharmacy,Chengdu Uni Institute of Orthopaedics,First Affiliated Hospital of General Hospital of PLA,Beijing 100048,China Department of Pharmacy,302 PLA Hospital of China,Beijing 100039,China Department of Outpatient,302 PLA Hospital of China,Beijing 100039,China State Key Laboratory of Virology,College of Life Science,Wuhan University,Wuhan 430072,China
国内会议
成都
英文
553-562
2013-11-01（万方平台首次上网日期，不代表论文的发表时间）