Modeling a murine model of IgE-mediated Qingkailing injection anaphylaxis
Qingkailing injection (QKLI) is a classic compound Traditional Chinese Medical injection (TCMI).It can cause anaphylatic shock and death in clinic.Modeling a murine model of Qingkailing injection anaphylaxis appears to be essential.In this study,for the first time,we used several strategies to prepare cBSA and QKLI-cBSA conjugate.The nBSA,cBSA and QKLI-cBSA conjugate were separated by cationic exchange method and also identified by UV-Vis and FT-IR spectrometry.Balb/c mice were then treated with subcutaneous injection of QKLI-cBSA plus alum.Serum were analyzed for total IgE and histamine levels by ELISAs,respectively.Lung reactions were evaluated by analyzing lung pathologic changes.QKLI-cBSA group mice were significantly and successfully sensitized.QKLI-cBSA conjugate significantly increased total IgE and Histamine in Balb/c mice serum.Moreover,histological examination of QKLI-cBSA conjugate treated mice showed acute injury with pulmonary alveoli and injury of trachea,as well as neutrophilic cell infiltration.In a word,we successfully established a murine model of IgE-mediated Qingkailing injection anaphylaxis.This model should provide a useful tool for detecting allergens in QKLI and for exploring new allergens-detecting approaches of other TCMIs.
Qingkailing injection murine IgE-mediated anaphylaxis QKLI-cBSA conjugate
Guoping Liao Wenshi Li Shuai He Zhongyi Zhang
Dept.of Pharmacy,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,PR China
国内会议
广州
英文
225-238
2012-01-07（万方平台首次上网日期，不代表论文的发表时间）