Serum long non-coding RNA uc022bqs.1 is a potential biomarker for early diagnosis of human coronary heart disease
Objectives:To determine the lncRNA expression profile in serum of coronary heart disease(CHD)patients and its potential clinical value.Methods:The difference of lncRNA expression profile between CHD patients and normal individuals was measured by lncRNA microarray.QRT-PCR was used to confirm the expression of representative lncRNA in 30 cases of normal control(NC),30 cases of stable angina pectoris(SAP),42 cases of unstable angina pectoris(UAP)and 30 cases of acute myocardial infarction(AMI).The relationship between their levels and pathological factors of patients with CHD was explored.A receiver operating characteristic(ROC)curve was adopted to verify the diagnostic efficiency of the selected lncRNAs.Results:LncRNA expression profile showed 166 lncRNAs with expression alteration eight fold or above.The up-regulated and down-regulated candidate lncRNAs were 105 and 61(P<0.005),respectively.The expression of serum uc022bqs.1 verified by qRT-PCR was consistent with the microarray analysis.Using ROC curves analysis,serum uc022bqs.1 could discriminate the SAP,UAP and AMI from NC and the areas under curve(AUC)was 0.796(95%CI:0.681~0.911),0.821(95%CI:0.718~0.924),0.846(95%CI:0.795~0.932),respectively.For discriminating the CHD patients from NC,the AUC was 0.895(95%CI:0.824~0.965),and the sensitivity and specificity were 79.5% and 83.3%,respectively.Conclusions:Serum uc022bqs.1 may serve as a potential marker for the early diagnosis of coronary heart diseases.
Coronary heart disease long noncoding RNAs serum diagnosis
张明娇 李珉珉
广州市暨南大学附属第一医院
国内会议
贵阳
英文
404-412
2016-07-01（万方平台首次上网日期，不代表论文的发表时间）